Revealing Translation Mechanisms in vivo
We have developed an improved ribosome footprint profiling methodology that allows us to determine ribosome state at individual codons genome-wide. With this new technology we are exploring the ribosome signals that activate MAPK signaling pathways to regulate translation.
Using ribosome profiling, CRISPR screening, and high-throughput chemical probing we are characterizing the activities of read-through drugs being developed for genetic disorders.
Ribosome states can be distinguished by the length of the mRNA fragments that they protect from RNase. Wu et al. Molecular Cell 2019
